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antimicrobial-resistance-prediction lineage-classification mycobacterium-tuberculosis
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Publication:10.1128/mra.00032-26 PDF: Available

Mycobacteria Amplicon Sequencing Tool: automated resistance prediction and lineage classification for Mycobacterium tuberculosis

Idowu B. Olawoye, Maxim Fedorov, Robert A. Petit III, Jennifer L. Guthrie

Abstract

ABSTRACT The Mycobacteria Amplicon Sequencing Tool ( https://github.com/guthrielab/MAST ) is a modular Nextflow pipeline for antimicrobial resistance prediction and lineage classification of Mycobacterium tuberculosis from amplicon or whole-genome sequencing data sets. The workflow automates read processing, variant calling, and annotation to produce standardized, human-readable reports in DOCX and TSV format.

Citation

Olawoye, I. B., Fedorov, M., Petit, R. A., 3rd, Guthrie, J. L. Mycobacteria Amplicon Sequencing Tool: automated resistance prediction and lineage classification for Mycobacterium tuberculosis. Microbiology Resource Announcements 15(6), e0003226-e0003226 (2026).